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Production of Cgtase from Bacillus Subti: Kumaraswamy, Bhargav

cgt, CGTase, Differential display, Symbiosis, Nostoc, Gunnera. Nationell ämneskategori. Mikrobiologi. Forskningsämne. The cyclization mechanism of cyclodextrin glycosyltransferase (cgtase) as revealed by a γ-cyclodextrin-cgtase complex at 1.8-å resolutionThe enzyme  ent amino acids residues of cyclodextrin glucanotransferase (CGTase) from. Archaeoglobus fulgidus in order to study the effects of those mutants on the catalytic  Production of CGTase from Bacillus subtilis. Bhargavi Kumaraswamy.

Cgtase

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1996-01-01 · This chapter discusses the purification and action of cyclodextrin-producing enzyme (CGTase). Purification of macerans CGTase can be accomplished by following steps: Starch adsorption and desorption, column chromatography on diethylaminoethyl-cellulose, and crystallization. By use of the crystal CGTase preparation, the action of the enzyme is studied. Purpose γ-Cyclodextrin glycosyltransferase (γ-CGTase) catalyzes the biotransformation of low-cost starch into valuable γ-cyclodextrin (γ-CD), which is widely applied in biotechnology, food, and pharmaceutical industries. However, the low specificity and activity of soluble γ-CGTase increase the production cost of γ-CD, thereby limiting its applications.

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2 344 4 a- Bacillus megaterium B. macerans CGTase 22 C-2, C-3, C-4 5) CGTase 6) , ) 10 8) 1, 3 Suzuki B. stearothermophilus CGTase Rhizopus sp. Phase 2: CGTase-catalyzed elongation of carboxylated alkyl glycosides - Design a set of experiments - Analyze data from HPLC, UV, LC-MS, NMR - Scale-up 219, nr 2, s. 181-185. Nyckelord [en].

Cgtase

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Cgtase

2 344 4 a- Bacillus megaterium B. macerans CGTase 22 C-2, C-3, C-4 5) CGTase 6) , ) 10 8) 1, 3 Suzuki B. stearothermophilus CGTase Rhizopus sp. Phase 2: CGTase-catalyzed elongation of carboxylated alkyl glycosides - Design a set of experiments - Analyze data from HPLC, UV, LC-MS, NMR - Scale-up 219, nr 2, s. 181-185. Nyckelord [en]. cgt, CGTase, Differential display, Symbiosis, Nostoc, Gunnera.

Cgtase

Unreviewed-Annotation score: -Protein inferred from homology i. Function i Cofactor i.
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Summary: The cyclomaltodextrin glucanotransferase (CGTase, EC 2.4.1.19) gene from the alkalophilic Bacillus sp.

In addition, CGTase production is highly dependent on the strain, medium composition and culture conditions. Objectives: Cyclodextrin glucanotransferase (CGTase) is a multifunctional industrial enzyme, which undergoes cyclization reaction to converts starch into Cyclodextrin (CD). Due to their potential properties, CDs had been discovered to more.
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Screening och val av medelkomponenter för cyklodextrin

In addition, we have revealed the key do-mains responsible for its hydrolytic activity and resistance induction. Further experiments demonstrated that CGTase is a potential func - Cyclodextrin glycosyltransferase (CGTase, EC 2.4.1.19) is a carbohydrate‐active enzyme that belongs to glycosyl hydrolase family 13. CGTase can catalyse hydrolysis reactions, cleaving the linkages in the starch molecule (van der Veen et al., 2000). Publisher Summary This chapter discusses the purification and action of cyclodextrin-producing enzyme (CGTase).


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2014). CGTases mainly perform three different reactions in addition to hydrolysis, namely cyclization, disproportionation and coupling. cgtase - Cyclodextrin glycosyltransferase - Anaerobranca gottschalkii - cgtase gene & protein UniProtKB - Q5ZEQ7 (Q5ZEQ7_9FIRM) γ-Cyclodextrin glycosyltransferase (γ-CGTase) catalyzes the biotransformation of low-cost starch into valuable γ-cyclodextrin (γ-CD), which is widely applied in biotechnology, food, and pharmaceutical industries.